Composite

Part:BBa_K310006:Design

Designed by: Erin Borchardt   Group: iGEM10_UIUC-Illinois   (2010-09-24)
Revision as of 19:20, 24 September 2010 by Eborcha2 (Talk | contribs) (→‎Design Notes)

ArsR under the control of J23100, includes RBS B0034 and terminator B0015


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 199


Design Notes

Site directed mutagenesis was performed in the coding region of ArsR due to the presence of XbaI site. This changed the coding sequence AGATCT to AGGTCT.

Source

I used PCR to isolate ArsR from E. coli K-12 MG1655 genome and attached B0015, B0034 and J23100 from the parts registry.

References