Composite

Part:BBa_K310006:Design

Designed by: Erin Borchardt   Group: iGEM10_UIUC-Illinois   (2010-09-24)
Revision as of 19:15, 24 September 2010 by Registry (Talk | contribs)

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ArsR under the control of J23100, includes RBS B0034 and terminator B0015


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 199


Design Notes

I needed to perform site directed mutagenesis in the coding region of ArsR due to the presence of XbaI site. This changed the coding sequence AGATCT to AGGTCT.


Source

I used PCR to isolate ArsR from E. coli K-12 MG1655 genome and attached B0015, B0034 and J23100 from the parts registry.

References