Part:pSB1A10:Design

The process for building pSB1A10,was:

1. Build I13534 using normal BB construcion.
2. Insert I13534 into psb1A3 plasmid
3. Scar out the BB prefix and suffix using Part:I13450
4. Re-insert a BB prefix/suffix into the center of I13534 using primers and around-the-horn PCR.

Some design notes:

1. The inclusion of the 'barcode' was unintentional (it was attached to Part:E1010), it has no (intended) function.
2. The "spacer artifacts" at position 22 and 5225 are artifacts of the construction process for inserting the BB restriction sites into the center of the plasmid. If you look at Part:I13534 you will see the device we buil