Part:BBa_K385002:Experience

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Applications of BBa_K385002

The MS2 coat protein reading frame was fused in-frame to GFP to make a translational fusion. It was placed under control of the yeast GAL1 promoter (BBa_J63006), and transformed into yeast in the single copy shuttle vector pRS415.

The transformants were grown overnight in synthetic defined medium containing 2% w/v galactose, and observed usin a fluorescence microscope optimised for GFP visualisation (Figure 1).

A control culture of the same transformant was grown using glucose as the carbon source; these conditions do not activate the GAL promoter. The results (Figure 2) show no GFP fluorescence.

Overall the results indicate that the MS2 coat protein can be successfully expressed as a protein fusion with other standard parts. Media:N25 + Gal-3.tif

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