Regulatory

Part:BBa_K431009

Designed by: David Joseph Sexton Jr.   Group: iGEM10_Georgia_State   (2010-09-21)
Revision as of 21:08, 21 September 2010 by Registry (Talk | contribs)

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glyceraldehyde 3-phosphate dehydrogenase promoter (pGAP)

This promoter is responsible for the transcription of glyceraldehyde 3-phosphate dehydrogenase in Pichia pastoris. Because this is a key enzyme for glycolysis, it is a strong constitutive promoter. This promoter is one of the most common alternatives to pAOX1 for heterologous protein production in P. pastoris. It is advantageous because its use obviates the hazards and expenses involved with using methanol and still provides high yields of protein.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 245


[edit]
Categories
//chassis/eukaryote/pichia
//promoter
Parameters
None