Regulatory

Part:BBa_K368001

Designed by: Jara Radeck   Group: iGEM10_LMU-Munich   (2010-09-21)
Revision as of 14:28, 21 September 2010 by Registry (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)


minimal CMV Promoter with TRE (tet responsible element)

CMV promoter induceable by Doxycycline. Only works in special Tet-On cell lines (stable transfected with pTet-On-Advanced Vector by clontech)! Also works in special Tet-Off cell lines (stable transfected with pTet-Off-Advanced Vector by clontech), but then the promoter will be inactivated by Doxycycline.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None