Generator

Part:BBa_K358010:Design

Designed by: Ken Kajita   Group: iGEM10_Kyoto   (2010-09-19)
Revision as of 08:05, 19 September 2010 by V08ken (Talk | contribs) (→‎Design Notes)

anti-killer gene with GFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 962


Design Notes

Made point mutation and deletion PCR on S gene to prepare this part.

Source

λ -Hind Ⅲ digest (product of TaKaRa Bio) and BBa_E0240

References