Generator

Part:BBa_K358010:Design

Designed by: Ken Kajita   Group: iGEM10_Kyoto   (2010-09-19)
Revision as of 06:31, 19 September 2010 by Registry (Talk | contribs)

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anti-killer gene with GFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 962


Design Notes

We made point mutation and deletion PCR on S gene to prepare this part.


Source

λ -Hind Ⅲ digest (product of TaKaRa Bio) and BBa_E0240

References