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Part:BBa_K358005:Design

Designed by: Ken Kajita   Group: iGEM10_Kyoto   (2010-09-17)
Revision as of 19:11, 17 September 2010 by V08ken (Talk | contribs) (New page: __NOTOC__ <partinfo>BBa_K358005 short</partinfo> <partinfo>BBa_K358005 SequenceAndFeatures</partinfo> ===Design Notes=== We took this gene by PCR from λ -Hind Ⅲ digest, which is the ...)

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lambda lysis cassette [SRRz]


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We took this gene by PCR from λ -Hind Ⅲ digest, which is the product of TaKaRa Bio. However, there is a mutation on S gene and it's not the wild-type. So, after, we done the point mutation PCR and prepared the wild-type DNA.

Source

λ -Hind Ⅲ digest(product of TaKaRa Bio), BBa_B0015


References

C. Chang, K. Nam, and R. Young, “S gene expression and the timing of lysis by bacteriophage lambda,” J. Bacteriol., vol. 177, Jun. 1995, pp. 3283-3294.