Generator

Part:BBa_K325219

Designed by: Bill Collins and Emily Knott   Group: iGEM10_Cambridge   (2010-09-15)
Revision as of 18:31, 15 September 2010 by Registry (Talk | contribs)

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Red Firefly Luciferase and LRE (under pBAD)
L. Cruciata
(E. coli optimised)

This part generates a red-mutant of the luciferase from the Japanese firefly (L.cruciata). It also generates the luciferin regenerating enzyme. When luciferin and D-cysteine are added this BioBrick should give sustained light output. With simply luciferin it will give a burst of light output.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
    Illegal NheI site found at 3278
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
    Illegal BamHI site found at 2169
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
    Illegal AgeI site found at 2118
    Illegal AgeI site found at 3820
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961
    Illegal SapI.rc site found at 1550
    Illegal SapI.rc site found at 3334


[edit]
Categories
Parameters
None