Translational_Unit

Part:BBa_K322451:Design

Designed by: William Rostain   Group: iGEM10_Edinburgh   (2010-09-01)
Revision as of 16:54, 1 September 2010 by Registry (Talk | contribs)

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Photinus pyralis luciferase mutated for increased bioluminescence.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1281
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1417
    Illegal NgoMIV site found at 1458
    Illegal AgeI site found at 1150
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Wild type sequence with three mutations which increase relative bioluminescence.

Reference Fujii, H., Noda, K., Asami, Y., Kuroda, A., Sakata, M. & Tokida, A. (2007). Increase in bioluminescence intensity of firefly luciferase using genetic modification. Analytical Biochemistry 366, 131-136.


Source

DNA was synthesised to have the same amino acids as the wild type except for the three mutations.


References