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Part:BBa_J70628:Design

Designed by: Joseph Lynch   Group: Knight Lab   (2010-08-11)
Revision as of 16:47, 16 August 2010 by Jolynch (Talk | contribs) (→‎Design Notes)

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RFC12 Sce VMA Intein Internal Domain


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 486
    Illegal BamHI site found at 1339
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1195
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Primers: 

 GTTTCTT C GAATTC GCGGCCGC T ACTAGT TGCTTTGCCAAGGGTACCAATGTTTTAATG, int-F
 GTTTCTT C CTGCAG CGGCCGC T GCTAGC TGCATGGACGACAACCTGGGATC, int-R

Source

NEB plasmid: [http://www.neb.com/nebecomm/products/productN6702.asp pTYB2]


References

<biblio>

  1. Kimple pmid=18429272
  2. Terpe pmid=12536251
  3. Perler pmid=8165123
  4. Perler pmid=9092614
  5. Chong pmid=8703028

</biblio>