Composite

Part:BBa_K389011

Designed by: Jonas Aretz   Group: iGEM10_Bielefeld-Germany   (2010-08-10)
Revision as of 18:23, 10 August 2010 by Jaretz (Talk | contribs)

VirA screening device

This part contains:

- a mutated virG response regulator from the virA/G receptor system from Agrobacterium tumefaciens which works in Escherichia coli without an rpoA gene from A. tumefaciens

- a kanamycin resistance under the control of a vir promotor

This device is used to screen virA receptor mutants. The virA receptor is mutated in the BBa_K389010 BioBrick. The better the receptor detects a substance, the more virG is phosphorylated and the stronger the kanamycin resistance from this BioBrick is expressed. The BioBricks BBa_K389010 and BBa_K389011 have to be used together in one cell.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//cds/selectionmarker/antibioticresistance
//cds/transcriptionalregulator/activator
//chassis/prokaryote/ecoli
//classic/measurement/uncategorized
Parameters
None