Generator
virA

Part:BBa_K389010

Designed by: Jonas Aretz   Group: iGEM10_Bielefeld-Germany   (2010-08-10)
Revision as of 18:07, 10 August 2010 by Registry (Talk | contribs)

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VirA receptor (expressed)

This BioBrick contains a virA receptor from Agrobacterium tumefaciens C58 under the control of a constitutive promotor.

We used it to mutate virA by error-prone PCR. For screening the mutants BBa_K389011 is also needed.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 647
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1632
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1768


[edit]
Categories
//cds/membrane/receptor
//cds/receptor
//chassis/prokaryote/ecoli
//classic/generator/uncategorized
//classic/signalling/sender
Parameters
None