Tag
Part:BBa_J70620
Designed by: Joseph Lynch Group: Knight Lab (2010-08-05)
RFC12 Maltose Binding Protein Internal Domain [malE]
This Maltose-binding protein is designed for either N-terminal or C-terminal fusions. MBP fused proteins can be purified in one step affinity chromatography using a cross-linked amylose matrix and then eluted with 10 mM maltose. Note that the amylose matrix can only be regenerated, in most cases, up to five times. The large size of this tag can also affect the fused protein.
MBP fusions often have higher levels of expression and solubility that the native untagged protein, with typical yields of up to 40 mg/liter culture.
Usage and Biology
Common tag information:
- Matrix: Cross-linked Amylose
- Yield/Purity: High yield, >70% purity
- # Steps: One step
- Fusion Location: Internal
- Affects Function?: Possibly
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 357
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 55
[edit]
Categories
Parameters
//proteindomain/affinity
//proteindomain/internal
//proteindomain/internal
| None |