Plasmid_Backbone

Part:BBa_K359201:Design

Designed by: George Zarubin   Group: iGEM10_Waterloo   (2010-07-28)
Revision as of 17:58, 28 July 2010 by Yazarubi (Talk | contribs) (→‎Source)

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pSB3K3-S-rbsRFP-P


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a suffix.
    Illegal SpeI site found at 2
    Illegal PstI site found at 885
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 3598
    Illegal NheI site found at 2253
    Illegal SpeI site found at 2
    Illegal PstI site found at 885
    Illegal NotI site found at 878
    Illegal NotI site found at 3604
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 3598
    Illegal XhoI site found at 1046
    Illegal XhoI site found at 1889
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found at 3598
    Plasmid lacks a suffix.
    Illegal SpeI site found at 2
    Illegal PstI site found at 885
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found at 3598
    Plasmid lacks a suffix.
    Illegal XbaI site found at 3613
    Illegal SpeI site found at 2
    Illegal PstI site found at 885
    Illegal AgeI site found at 581
    Illegal AgeI site found at 693
    Illegal AgeI site found at 2339
    Illegal AgeI site found at 2662
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI.rc site found at 3367


Design Notes

Non-standard suffix of BBa_J6100 is inherited.

Source

BBa_J61002-BBa_J23101, pSB3K3-BBa_I20260 from 2010 Spring Distribution

References