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Coding

Part:BBa_K325000:Design

Designed by: Theo Sanderson   Group: iGEM10_Cambridge   (2010-07-26)
Revision as of 14:08, 26 July 2010 by Theos (Talk | contribs) (→‎Source)

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Luciferin Regenerating Enzyme from Photinus Pyralis


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 364
    Illegal AgeI site found at 409
    Illegal AgeI site found at 880
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We removed a PstI site


Source

http://www.ncbi.nlm.nih.gov/nucleotide/14331151?report=genbank&log$=nucltop&blast_rank=1&RID=4EUAXMJW01R optimised with OPTIMIZER, planned to synthesise.

References