Coding

Part:BBa_J70613

Designed by: Joseph Lynch   Group: Knight Lab   (2010-07-22)
Revision as of 15:21, 22 July 2010 by Jolynch (Talk | contribs)

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RFC12 GFP coding region

This is an RFC12 protein coding sequence, i.e. it has both a start codon and a double stop codon. Unlike BBa_J70595, this part will actually cause your cells to turn green if you put a promoter+rbs (such as BBa_J70608) in front of it.

Note The assembly scars shown below are incorrect as of July 22, 2010. The correct scars should be RFC12 scars of GCTAGT.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 652


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Categories
Parameters
None