Coding

Part:BBa_J72016:Design

Designed by: Jennifer Brophy   Group: Anderson Lab   (2010-07-15)
Revision as of 21:07, 15 July 2010 by Jennbrophy (Talk | contribs) (→‎Wobble construction of Bmll56 {rbs_at_10000.6XHis>})

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{Bmll56} in BBb


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]



Source

Overlap Extension PCR (Wobble construction)

Wobble construction of Bmll56 {rbs_at_10000.6XHis>}

Wobble mll56/mll57                (88, EcoRI/BamHI)
Sub in pBca9523-Bca1144#5           (EcoRI/BamHI, 2472+1224, L)
Product is pBca9523-Bmll56         {rbs_at_10000.6XHis>}
----
mll56    Forward wobble for {rbs_at_10000.6XHis>} (Bmll56)

CCATAGAATTCATGAGATCTCTACAACACCTAGCATCTAAGAAGTTACATATGGATTC

mll57    Reverse wobble for {rbs_at_10000.6XHis>} (Bmll56)

CGTTAGGATCCGTGATGGTGATGATGGTGAGAATCCATATGTAACTTCTTAG


References