Device
"Lock"

Part:BBa_K318030:Design

Designed by: Justin Vrana, Nate Pantalone   Group: iGEM10_Wisconsin-Madison   (2010-07-13)
Revision as of 19:51, 13 July 2010 by Registry (Talk | contribs)

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lox66 + rpCons + hixC + lox71 + SapI + T + T + Hin enhancer + HindIII + hixC


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 43
    Illegal NheI site found at 66
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 162


Design Notes

Note that the constitutive promoter is oriented to express whatever gene is downstream of the final hixC site.


Source

From Synthesis

References