Generator

Part:BBa_I739001:Experience

Designed by: Christian Kemmer, Stefan Luzi   Group: iGEM07_ETHZ   (2007-09-27)
Revision as of 22:23, 7 July 2010 by Pettigr7 (Talk | contribs) (→‎User Reviews)

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This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_I739001

User Reviews

UNIQ56f35860acc0a550-partinfo-00000000-QINU UNIQ56f35860acc0a550-partinfo-00000001-QINU

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Michael Pettigrew

I used this part for constitutive expression of tetR. After transforming the device DNA from the 2010 iGEM distribution into DH5a E.coli, I miniprepped the sample, cut with EcoRI and PstI, and ran on a gel. The insert was ~2400bp, and the plasmid was ~3000bp. This is quite a bit more than the ~900bp the part is supposed to be. I decided to instead use Q04400 and add a constitutive promoter in front. I did not test to see whether I739001 still expressed tetR.