Coding

Part:BBa_K343000

Designed by: Lars Christian Lund   Group: iGEM10_SDU-Denmark   (2010-07-02)
Revision as of 11:39, 3 July 2010 by Lclund (Talk | contribs)

Mutated flagella master regulator (FlhDC mut).

The master regulator operon for flagellum synthesis. Produces transcriptional factors for Class II flagellar regulon genes. To make the part conform to the iGEM assembly standards we introduced a silent mutation, in an unintended restriction site, at BP 822 from t to c, so that Pst1 would not cleave the DNA there.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 609
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None