Coding
PS

Part:BBa_K343003:Design

Designed by: Christian Kurtzhals   Group: iGEM10_SDU-Denmark   (2010-07-02)
Revision as of 16:14, 2 July 2010 by Lclund (Talk | contribs) (→‎Design Notes)

NpSopII-NpHtrII-StTar (M-fusion)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 1783
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 73
    Illegal NgoMIV site found at 331
    Illegal AgeI site found at 1585
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1027
    Illegal BsaI.rc site found at 1300
    Illegal SapI site found at 801
    Illegal SapI.rc site found at 1801


Design Notes

NpSopII-NpHtrII-EcTSR fusion-chimera protein sequence from Jung K-H, Spudich EN, Trivedi VD and Spudich JL; An Archaeal Photosignal-Transducing Module Mediates Phototaxis in Escherichia coli; Journal of Bacteriology, Nov. 2001, p. 6365–6371. [http://jb.asm.org/cgi/content/full/183/21/6365]

The first 224 amino acid residues come from the NpSopII gene, encoding a bluelight photon receptor with 15 residues removed at the C-terminal end. The following 9 amino acids are a linker. the last part is HtrII fused with Tsr from E.Coli at the HAMP domain. The complex' first 125 amino acid residues come from HtrII and the remaining 279 from Tsr.

Source

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References