Part:BBa_K249019:Experience

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User Reviews

UNIQe5275630adf330d1-partinfo-00000000-QINU UNIQe5275630adf330d1-partinfo-00000001-QINU

When sequencing the stock we had from 2009 our team (Lethbridge) found a RBS mutation. We attempted to overexpress the protein and found that it would not. We do not know if this is the same for the kits that were sent out this year or not. We recommend that you sequence the part before use and if it has the same mutation do not use directly but mutate the mutation out. The mutation we found is as follows:

             Mms6 7 VF Prefix   TCCCGACTGGAAAGCGGG-NNANAGNGCAACGCAATTAATGTGAGTTAGCTCACTCATTA
Mms6 7 VR A-Suffix (reversed)   TCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATGTGAGTTAGCTCACTCATTA
             Mms6 8 VF Prefix   TCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATGTGAGTTAGCTCACTCATTA
Mms6 8 VR A-Suffix (reversed)   TCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATGTGAGTTAGCTCACTCATTA
                         mRBS   TCAC-ACAGGAAAG----------------------------------------------