Tag

Part:BBa_K416003:Design

Designed by: John Phair   Group: iGEM10_NYU   (2010-06-23)
Revision as of 20:26, 23 June 2010 by Jphair (Talk | contribs) (→‎References)

Yeast Secretion Tag


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

There was an illegal Xbal1 site at bp 101 and we removed it through altering the primers necessary for biobricking the sequence.


Source

This part was sent to use from Dr. Sheldon Park of the University of Buffalo.

References

Gene. 1991 Oct 15;106(2):267-71. Secretion of human epidermal growth factor from Saccharomyces cerevisiae using synthetic leader sequences. Clements JM, Catlin GH, Price MJ, Edwards RM.