Protein_Domain

Part:BBa_J70595:Design

Designed by: Joseph Lynch   Group: Knight Lab   (2010-06-23)
Revision as of 14:46, 23 June 2010 by Jolynch (Talk | contribs) (→‎Design Notes)

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RFC12 Green Fluorescent Protein


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 641


Design Notes

Amplified via colony PCR directly from the BBa_E0040 cell stock. Note that both the ATG and double TAA codons were removed.

Forward Primer: 

 5' GTTTCTT C GAATTC GCGGCGC T ACTAGT CGTAAAGGAGAAGAACTTTTCACTG 3', GFP-F

Reverse Primer: 

 5' GTTTCTT C CTGCAG CGGCCGC T GCTAGC TTTGTATAGTTCATCCATGCCATGTG 3', GFP-R

Source

BBa_E0040

References