Protein_Domain

Part:BBa_J70594:Design

Designed by: Joseph Lynch   Group: Knight Lab   (2010-06-18)
Revision as of 19:41, 18 June 2010 by Jolynch (Talk | contribs) (→‎Design Notes)

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RFC12 TAATAA Tail Domain


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Made with synthetic oligos: 

5' AATTC GCGGCGC T ACTAGT TAATAA GCTAGC A GCGGCCG CTGCA 3', Tail-F
5' GCGGCCGCTGCTAGC TTATTA ACTAGTAGCGCCGC G 3', Tail-R

Note that both primers were ordered phosphorylated. An alternative is to phosphorylate the primers yourself with a kinase.

Source

Common Knowledge

References