RBS

Part:BBa_J70591:Design

Designed by: Joseph Lynch   Group: Knight Lab   (2010-06-18)
Revision as of 16:11, 18 June 2010 by Jolynch (Talk | contribs) (Design Notes)

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RFC12 Elowitz RBS


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Designed with synthetic oligos:

5' AATTC GCGGCGC T ACTAGT AAAGAGGAGAAA GCTAGC A GCGGCCG CTGCA 3', RBS-F
5' GCGGCCGCTGCTAGC TTTCTCCTCTTT ACTAGTAGCGCCGCG 3', RBS-R

Note that both primers were ordered phosphorylated. An alternative is to phosphorylate the primers yourself with a kinase.

Source

Part BBa_B0034

References