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Part:BBa_J70500

Designed by: Tom Knight   Group: Knight Lab   (2009-09-13)
Revision as of 23:32, 29 May 2010 by Tk (Talk | contribs)

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pWV01 broad host range plasmid origin and rep protein

pWV01 broad host range plasmid origin and rep protein. pWV01 replicates by rolling circle replication in a very broad range of bactera, including both gram positive and gram negative species. This part is the a stripped down plasmid origin for constructing a Biobrick plasmid.

For use with mycoplasma species, the repA (orfD) seqeunce terminates in a TGA stop codon, which will be interpreted as tryptophan. This may need to be modified for use in the mycoplasma and mesoplasma species.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//chassis/prokaryote/mflorum
Parameters
None