Part:BBa_K237000

RIP - Quorum sensing inhibitor of Staphylococcus spp. (Including MRSA)

RIP or RNAIII inhibiting peptide is a heptapeptide capable of severely reducing the Quorum sensing(QS) abilities of Staphylococcus species [1]. RIP effectively works as an antibiotic in its inhibition of bacterial biofilm-formation. It is important to note that even though it has the effect of an antibiotic [1] on bacterial load, the mechanism is a novel one, and doesn't appear to induce bacterial resistance [1].

RIP has the sequence YSPXTNF(where X can be a cysteine, a tryptophan, or a modified amino acid) and has been tested extensively in a synthetic (as in chemical synthesis) form for ability to inhibit bacterial infections in mice [1] and biofilm formation on human cells [2]. References will come later.

This is a pure RIP Brick, it contains no signal sequence and we can not expect nor verify that it will be exported by our Coli's

References

[1] Suppression of Drug-Resistant Staphylococcal Infections by the Quorum-Sensing Inhibitor RNAIII-Inhibiting Peptide. Journal of Infectious Diseases [serial on the Internet]. (2004, July 15), [cited October 21, 2009]; 190(2): 318-320. Available from: Academic Search Premier. [http://www.ncbi.nlm.nih.gov/pubmed/15216467]

[2] Prevention of Staphylococcus aureus biofilm on dialysis catheters and adherence to human cells. Balaban N, Gov Y, Bitler A, Boelaert JR. Kidney Int 2003; 63:340–5. [http://ncbi.nlm.nih.gov/pubmed/12472801]

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]