Part:BBa_R0010

promoter (lacI regulated)

This part is an inverting regulator sensitive to LacI and CAP.

It contains two protein binding sites. The first binds the CAP protein, which is generally present in E.coli and is asocciated with cell health and availability of glucose. The second binds LacI protein.

• In the absence of LacI protein and CAP protein, this part promotes transcription.
• In the presence of LacI protein and CAP protein, this part inhibits transcription.
• LacI can be inhibited by [http://openwetware.org/wiki/IPTG IPTG].
• LacI is coded by BBa_C0010

Usage and Biology

This is a direct copy of bases 0365739 through 0365540 of the E. coli K-12 MG1655 genome, Genbank NC_000913 in reverse complement form. It is the natural promoter for the LacZYA operon. It includes the tail end of the LacI gene coding region, but no promoter region for that partial gene.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Attention! This sequence is indeed 0365739 through 0365540 of the E. coli K-12 MG1655 genome, but encodes part of lacI repressor! Please verify it.