Translational_Unit

Part:BBa_J70565

Designed by: Joseph Lynch   Group: Knight Lab   (2010-03-30)
Revision as of 19:26, 30 March 2010 by Jolynch (Talk | contribs)

luxAB Fusion gene, Xenorhabdus luminescens

This part includes a fusion of the coding sequences for the luxA and luxB genes of the organism Xenorhabdus luminescens, along with luxA's native ribosomal binding site.

The coding regions provided by K216008,luciferase LuxAB of Xenorhabdus luminescens, were used in the fusion gene. Via a standard fusion PCR reaction luxA's stop codon was removed and the intervening nucleotides were replaced with a 10 amino Gly-Ser linker. In addition, the nonfunctional SpeI cut site in K216008 was fixed so that the part could be assembled with others in the future.

LuxAB codes for a dimer that in the presence of decanal, produces light. This decanal can either be provided in-vitro with n-Decanal, or in-vivo by the luxCDABE cassete (which will produce tetradecanal).


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 530
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 1049


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Categories
Parameters
None