Tag
HisTag

Part:BBa_J18909

Designed by: Raik Gruenberg   Group: Affiliates   (2010-01-15)
Revision as of 16:59, 26 January 2010 by Raik (Talk | contribs) (→‎Notes)

His tag (6xHis; E. coli - optimized)

Hexahistidine affinity tag.

Binds zinc, nickel, or cobalt affinity resins.

Notes

This part is identical to BBa_K157011 (for which the registry currently reports sequencing issues). The part is provided with prefix and suffix for RFC 25 BioBrick assembly (flanked by NgoMIV / AgeI).

See also:

Lab-internal ID: rg0022

References

Wikipedia article: [http://en.wikipedia.org/wiki/Polyhistidine-tag]


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//proteindomain/affinity
Parameters
None