Primer

Part:BBa_J18910

Designed by: Raik Gruenberg   Group: Affiliates   (2010-01-25)
Revision as of 09:53, 25 January 2010 by Raik (Talk | contribs)

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RFC 25 vector forward (suffix)

forward primer for vector linearization of any vector containing the RFC 25 Biobrick flanks, anneals to suffix covering AgeI and SpeI site 

   * Tm (oligocalc nearest neighbore, 500nM primer, 50mM salt) = 60 C
   * Tm (finnzyme, 500nM primer, 50mM salt) = 65
   * Tm (biophp, 500mM, 50mM, 1.5mM MgCl2) = 67.5

See also: J18911 for reverse

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 11
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 11
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 11
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 11
    Illegal AgeI site found at 1
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None