Primer

Part:BBa_J18911

Designed by: Raik Gruenberg   Group: Affiliates   (2010-01-25)
Revision as of 09:46, 25 January 2010 by Registry (Talk | contribs)

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RFC 25 vector reverse (prefix)

Primer for backbone linearization of RFC 25 construction vectors. Aligns to the RFC 25 suffix but pointing outward into the backbone rather than insert.

Annealing temperature: 68 C (in Phusion polymerase buffer)

Example vectors: pSB1AC3F, pSB1AK3F, pSB1AT3F, pMA

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 9
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 9
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 9
    Illegal NgoMIV site found at 1
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None