Part:BBa_K5248055

pACYCDuet-1-mCherry-mchI（6his)-EGFP-mchB(strep)

We inoculated 100 μL of each predator strain's overnight concentrated culture on LB agar plates, covered with soft LB agar containing prey strains. For the mcmA and mchB antimicrobial peptide expression plasmids, the prey strain was Escherichia coli DH5α, and the predator strains were E. coli Nissle 1917 DE3 and BL21 DE3. In this experiment, the first two plates showed predators expressing mcmA and mchB, with the prey sensitive to these antimicrobial peptides.

  < img src="https://static.igem.wiki/teams/5248/wet-lab/results/59.webp">  

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal EcoRI site found at 143
  Illegal PstI site found at 369
  Illegal PstI site found at 378
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 143
  Illegal NheI site found at 589
  Illegal PstI site found at 369
  Illegal PstI site found at 378
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 143
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal EcoRI site found at 143
  Illegal PstI site found at 369
  Illegal PstI site found at 378
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 143
  Illegal PstI site found at 369
  Illegal PstI site found at 378
  
• 1000
  COMPATIBLE WITH RFC[1000]