Regulatory

Part:BBa_K5380001:Experience

Designed by: Miles Anderson   Group: iGEM24_BYU-Provo   (2024-10-01)
Revision as of 13:47, 2 October 2024 by Mileskatahdin (Talk | contribs)


Arabidopsis Thaliana utilizes NIN-Like Proteins (NLPs), which undergo conformational changes in the presence of Nitrate (Konishi et al., 2013; Chardin et al., 2014). NLPs, having gone aforementioned conformations, will interact as transcription factors by binding to Nitrate Responsive Cis-Elements (NREs), thus increasing expression rates of downstream elements (Konishi et al., 2013; Chardin et al., 2014).

Applications of BBa_K5380001

Arabidopsis Thaliana utilizes NIN-Like Proteins (NLPs), which undergo conformational changes in the presence of Nitrate (Konishi et al., 2013; Chardin et al., 2014). NLPs, having gone aforementioned conformations, will interact as transcription factors by binding to Nitrate Responsive Cis-Elements (NREs), thus increasing expression rates of downstream elements (Konishi et al., 2013; Chardin et al., 2014). We used this part in our project as a promoter to up regulate downstream genes in response to the presence of nitrate in our plant system. We were unable to verify its efficiency in our plant, as we were unable to verify the insertion of the part, but its efficacy is well validated in previous publications.

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UNIQ3f21d516f80c712f-partinfo-00000000-QINU UNIQ3f21d516f80c712f-partinfo-00000001-QINU