Composite
Part:BBa_K5359001:Design
Designed by: Kevin Li Group: iGEM24_BostonU (2024-10-02)
SYFP2 RBS + SYFP2
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
RBS sequence calculated based on entire protein coding sequence.
Source
RBS sequence sourced from bioinformatic calculator. SYFP2 sequence sourced from Kremers et al.
References
1. Kremers, G.-J., Goedhart, J., van Munster, E. B., & Gadella, T. W. J. (2006). Cyan and yellow super fluorescent proteins with improved brightness, protein folding, and FRET Förster radius. Biochemistry, 45(21), 6570–6580. doi:10.1021/bi0516273