Coding

Part:BBa_K5173007

Designed by: Jiayi Chen   Group: iGEM24_EHSBNU   (2024-10-02)
Revision as of 13:36, 2 October 2024 by Registry (Talk | contribs)

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Clotting factor G beta subunit optimized for e.coli expression host

To accomodate the eukaryotic protein to the prokaryotic e.coli expression host and boost expression efficiency, we optimized the mature peptide sequence of its beta subunit using NovoPro ExpOptimizer.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 485
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None