Coding
Part:BBa_K5173006:Design
Designed by: Jiayi Chen Group: iGEM24_EHSBNU (2024-10-02)
Clotting factor G alpha subunit optimized for e.coli expression host
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
We downloaded and optimizied the mature peptide sequence of the alpha subunit only because the prokaryotic expression host lacks protein modification mechanisms.
Source
The mature peptide sequence of the alpha subunit was downloaded from NCBI GenBank.