Plasmid

Part:BBa_K5515005:Design

Designed by: Aiman Dhiloon   Group: iGEM24_McMasterU   (2024-10-02)
Revision as of 13:25, 2 October 2024 by Registry (Talk | contribs)

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pASapI


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 2445
    Illegal XbaI site found at 3821
    Illegal PstI site found at 3833
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 4362
    Illegal NheI site found at 5791
    Illegal NheI site found at 6112
    Illegal PstI site found at 3833
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 3815
    Illegal XhoI site found at 3739
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 2445
    Illegal XbaI site found at 3821
    Illegal PstI site found at 3833
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 2445
    Illegal XbaI site found at 3821
    Illegal PstI site found at 3833
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1319
    Illegal BsaI site found at 3923
    Illegal BsaI.rc site found at 6518
    Illegal SapI.rc site found at 3800


Design Notes

if in e.coli must include a second promoter and terminator


Source

Saul Purton, University College London, August 2014

References