Plasmid

Part:BBa_K5368001

Designed by: Yifei Wu   Group: iGEM24_SCUT-China-B   (2024-10-02)
Revision as of 13:21, 2 October 2024 by Registry (Talk | contribs)

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pU57-Tyr-anti-CD3 scfv-CD86-mut IL-2

We first chose the tyrosinase promoter (Tyr), whose specificity is reflected in the fact that it is abundantly expressed only in melanoma cells. Then the sequences corresponding to αCD3, and CD86, who are the first and second signals for activating T cells, respectively, were connected. Finally we linked the DNA sequence of mut-IL2, which can recruit T cells and promote T cell proliferation. It can be understood that this is a gene editing drug that transforms melanoma cells into immune cells.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None