DNA

Part:BBa_K5443014:Experience

Designed by: Nick Coleman, Carmen Hawthorne   Group: iGEM24_MacquarieUni   (2024-09-27)
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Applications of BBa_K5443014

We showed that the ECH-Str enzyme from Streptomyces was functional in E. coli as part of a vanillin biosynthetic pathway. This was evidenced by detectable production of vanillin (0.2 ppm), determined by LC-MS analysis of our tested clone pMQ3C-11 (Fig. 1).

As functional ECH-Str is essential for vanillin production in this pathway, detectable presence of vanillin shows at least partial success of our construct pMQ3C-11.

Figure 1. LCMS analysis of compounds produced using pMQ3C-11.
The graph shows the detected presence of all 6 tested compounds, validating successful expression of all parts within pMQ3C-11, including ECH-Str.