Coding

Part:BBa_K5051002

Designed by: Yuk Ho Tsang   Group: iGEM24_PLKNPL-HK   (2024-10-01)
Revision as of 12:51, 2 October 2024 by Thegodofsheep (Talk | contribs)

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EST1

The EST1 gene from Aureobasidium pullulans EXF-150 encodes an esterase that is essential for the final assembly of the liamocin structure. After polyketide synthase synthesizes fatty acid chains, EST1 promotes an esterification process in which these fatty acid chains are attached to a single sugar alcohol molecule, usually mannitol or arabitol (Xue et al., 2020).

parts3.png

Fig 1. Structure predicted by ESMFold, colored by pLDDT (Lin et al., 2023)

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 445
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None