Coding

Part:BBa_K5051002:Design

Designed by: Yuk Ho Tsang   Group: iGEM24_PLKNPL-HK   (2024-10-01)
Revision as of 12:40, 2 October 2024 by Thegodofsheep (Talk | contribs) (→‎References)


EST1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 445
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Codon optimised for Saccharomyces cerevisiae



Source

Aureobasidium pullulans EXF-150

References

1. Lin, Z., Akin, H., Rao, R., Hie, B., Zhu, Z., Lu, W., Smetanin, N., Verkuil, R., Kabeli, O., Shmueli, Y., Costa, A. D. S., Fazel-Zarandi, M., Sercu, T., Candido, S., & Rives, A. (2023). Evolutionary-scale prediction of atomic-level protein structure with a language model. Science, 379(6637), 1123–1130. https://doi.org/10.1126/science.ade2574

2. Xue, S., Liu, G., Chi, Z., Gao, Z., Hu, Z., & Chi, Z. (2020). Genetic evidences for the core biosynthesis pathway, regulation, transport and secretion of liamocins in yeast-like fungal cells. Biochemical Journal, 477(5), 887–903. https://doi.org/10.1042/bcj20190922