Part:BBa_K5163001
Vanillin synthase under the control of the blue light-sensitive pDawn promoter
This section features a genetic construct containing the vanillin terpene synthase genes, which are driven by the pDawn promoter. The pDawn promoter is sensitive to blue light, allowing for precise, light-controlled expression of the target genes. When exposed to blue light, the system activates the production of key enzymes involved in the biosynthesis of vanillin.
The construct includes several important components: - *gltA* serves as a key indicator of the system's yield, helping to measure the efficiency of the biosynthetic process. - *fcs* (feruloyl-CoA synthetase) is responsible for catalyzing the conversion of ferulic acid into feruloyl-CoA, a crucial intermediate in the vanillin production pathway. - *ech* (enoyl-CoA hydratase) further converts feruloyl-CoA into vanillin, the final product.
By incorporating these genes under the control of the light-sensitive pDawn promoter, the system can precisely regulate vanillin production through the application of blue light.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 125
Illegal NotI site found at 2824 - 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 65
Illegal XhoI site found at 199
Illegal XhoI site found at 1309 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 186
Illegal NgoMIV site found at 450
Illegal NgoMIV site found at 928
Illegal NgoMIV site found at 1183
Illegal NgoMIV site found at 1246
Illegal NgoMIV site found at 1263
Illegal NgoMIV site found at 2253
Illegal AgeI site found at 2247 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 958
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