Part:BBa_K5121015
rebB N-terminal CGGGGS
Background
RebB encodes an 13 kDa protein which forms one of the main structural components of type 51 R bodies. This part is based off of the wildtype rebB part — BBa_K2912001 — uploaded by SZU-China in 2019, however, has been modified to be compatible with cysteine maleimide conjugation at the N-terminus. This is to allow for conjugation with entire R bodies — note that R bodies should be assembled and purified prior to conjugation with the constituent rebB monomers. Our team aimed to use conjugation to attach drugs onto R bodies for use in drug delivery.
Cysteine maleimide conjugation is a form of Michael addition, in which the thiol of the cysteine acts as a nucleophile to react with maleimide, forming a thiosuccinimide adduct. Through this reaction, drugs with maleimide groups can hence be reacted onto proteins with readily accessible cysteines.
Since wildtype rebB does not have any cysteines (and nor does RebA), maleimide conjugation provides an ideal conjugation method for orthogonal attachment onto R bodies. This part contains an N-terminal cysteine that has been added to facilitate N-terminal conjugation using cysteine maleimide addition. See the design page for more details.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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