Coding

Part:BBa_K5051000:Design

Designed by: Yuk Ho Tsang   Group: iGEM24_PLKNPL-HK   (2024-10-01)
Revision as of 12:27, 2 October 2024 by Thegodofsheep (Talk | contribs) (→‎References)


PKS1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1012
    Illegal BglII site found at 1504
    Illegal BglII site found at 4192
    Illegal BglII site found at 5371
    Illegal BglII site found at 5677
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 2939
    Illegal SapI.rc site found at 5579


Design Notes

It was codon optimized for Saccharomyces cerevisiae


Source

The PKS1 gene is from Aureobasidium pullulans EXF-150 genomic sequence

References

1. Xue, S., Liu, G., Chi, Z., Gao, Z., Hu, Z., & Chi, Z. (2020). Genetic evidences for the core biosynthesis pathway, regulation, transport and secretion of liamocins in yeast-like fungal cells. Biochemical Journal, 477(5), 887–903. https://doi.org/10.1042/bcj20190922