Coding

Part:BBa_K5382103

Designed by: yuyanyan chen   Group: iGEM24_HUBU-4-CHN   (2024-09-25)
Revision as of 12:25, 2 October 2024 by Chenyuyanyan (Talk | contribs)

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InaK-ice nucleoprotein

InaK is a kind of ice nucleoprotein of Pseudomonas syringae KCTC1832. Ice nucleoprotein is a kind of protein that can promote the formation of ice crystals in organisms. Its N-terminal domain can be tightly anchored to the surface of Escherichia coli Nissle1917 cells as an anchor moda, so as to enable surface display.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 631
    Illegal NgoMIV site found at 871
    Illegal NgoMIV site found at 1039
    Illegal AgeI site found at 426
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 601
    Illegal BsaI.rc site found at 1091
    Illegal SapI.rc site found at 235


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Categories
Parameters
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