Composite
Part:BBa_K5236020
Designed by: Hening Guo Group: iGEM24_BASIS-China (2024-10-01)
pET28a-pelB-BhrPETase(N205G)-6xHis
This composite part codes for the mutated BhrPETase N205G and expresses the enzyme in the extracellular space of chassis.
T7 promoter: used to regulate gene expression of recombinant proteins.
Lac operator: used to control gene expression by lactose.
RBS: the location to bind ribosome.
BhrPETase N205G: This sequence encodes for a mutated BhrPETase N205G and is derived from Escherichia coli.
pelB: the sequence codes for signal peptide which helps PETase exit the cell membrane.
6xHis: a tag for the purification of protein which focus in the production of recombinant proteins.
T7 terminator: terminates transcription.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 308
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 218
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 912
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Categories
Parameters
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